Figure 3
From: Inhibition of SLPI ameliorates disease activity in experimental autoimmune encephalomyelitis
SLPI has an inhibitory effect on TGF-β. A: 100,000 U937 cells were incubated for 16 h in 0.2 mL RPMI medium with or without SLPI. TGF-β expression was quantified by RealTime-PCR (**, p < 0.01 (Student's t-test)) B: Blood was drawn from SLPI- and OVA-immunized mice at the end of the experiment shown in Figure 2A. Total TGF-β was quantified after activation of latent TGF-β in the samples by hydrogen chloride treatment. Differences between samples from SLPI-immunized and OVA-mice immunized were tested for their statistical significance using a Student's t-test. (*, p < 0.05) C: Blood was drawn from SLPI- and OVA-immunized rats at day 14 after EAE induction. Total TGF-β has been quantified after activation of latent TGF-β in the samples by hydrogen chloride treatment. Activated TGF-β levels were determined by skipping the activation step. Differences of total and activated TGF-β levels between samples isolated from SLPI-immunized and OVA-immunized rats were tested for their statistical significance using a Student's t-test. (*, p < 0.05)