Figure 4

Protein levels of (A) 15-LOX, and (B) p450 epoxygenase (with representative immunoblots) in control, low- and high-dose LPS rat brain. Data are ratios of optical densities of protein to β-actin, expressed as percent of control and were compared using one-way ANOVA, mean ± SEM (n = 8). Representative brain transcription factor binding activities (DNA-protein complex) of (C) NF-κB p50 and (D) NF-κB p65 in control, low- and high-dose LPS rat brains. DNA binding activity was measured in brain nuclear extracts as described in Materials and Methods. Mean ± SEM (n = 8), **p < 0.01. Protein levels of (E) phospho-p38 MAPK (with representative immunoblots) in control, low- and high-dose LPS rat brain. Data are ratios of optical densities of protein to β-actin, expressed as percent of control. Comparisons between LPS groups were made using one-way ANOVA, mean ± SEM (n = 8), *p < 0.05.