Figure 3
Retinal glia activation is similar between WldStransgenic and wildtype rats after optic nerve crush and lens injury. (A) Retinal glia activation (measured as percentage increase in GFAP intensity compared to control; control set as 100% baseline) 14 days after either optic nerve crush alone or in combination with lens injury in adult wildtype and WldS transgenic rats. Significant differences are indicated by asterisk (P < 0.001 = ***). Cross-sections through adult wildtype or WldS retinae stained for presence of GFAP + astrocytes (arrowheads)/Müller glia (open arrowheads) from (B, C) control (untreated), or 14 days after (D, E) optic nerve crush, or (F, G) optic nerve crush in combination with lens injury. The RGC layer is marked in panel B by an asterisk. WT = Wildtype; ONC = Optic nerve crush; ONC + LI = Optic nerve crush + lens injury; Scale bars: 50 μm