Figure 5From: Decreased expression of extracellular matrix proteins and trophic factors in the amygdala complex of depressed mice after chronic immobilization stressThe expression of Igfbp2 and Igf2 in the amygdala was analyzed using IHC in the three groups; CTL-MI, STR-HI, and STR-LI mice. IHC showed specific decreased expression of Igfbp2 and Igf2 in the CeA nucleus of STR-HI mice (A). The fluorescence signal density was quantified using the NIH Image J program, and the strength of IHC was evaluated with one-way ANOVA and Tukey’s multiple comparison tests among groups. Igfbp2 (F = 13.52) and Igf2 (F = 53.25) showed statistically significant differences among groups with one-way ANOVA. Tukey’s post tests revealed that only the STR-HI mice had a significant decrease in two proteins in the CeA (B). Data are the means ± SEM (n = 3). a, P < 0.05 between CTL-MI and STR-HI mice; c, P < 0.05 between STR-HI and STR-LI mice. Scale bars are 200 μmBack to article page