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Figure 11 | BMC Neuroscience

Figure 11

From: A neurochemical map of the developing amphioxus nervous system

Figure 11

Double in situ hybridization of VAChT and VGAT.Whole mounts with anterior to the left and whole mount scale bars are 50 μm. A-D: Serial confocal fluorescence images of a 18-hr neurula. A: VAChT-positive neurons (green). B: VGAT-positive neurons (red). C: Merger of A and B. D: Magnification of the anterior neural tube shown in C. E,F: Lateral views of a 22-hr neurula. In E, the dark purple and red staining respectively show VAChT and VGAT expression. In F, the superimposition of brightfield and epifluorescence shows both VAChT-positive and VGAT-positive neurons.

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