Figure 8
From: A neurochemical map of the developing amphioxus nervous system
Overlapping expression of GAD and VGAT revealed by double in situ hybridization. Anterior is to the left and whole mount scale bars are 50 μm. A-C: Lateral views of a 16-hr neurula. A: GAD expression is detectable in three spots of the neural tube. B: Double-labeled embryo viewed by epifluorescence showing five pairs of VGAT-positive neurons. C: Merger of A and B showing the colocalization of GAD and VGAT in three anterior pairs of neurons. D,E: Lateral views of a 18-hr neurula. D: Double-labeled embryo. The dark brown staining shows co-expression of GAD and VGAT, while the red staining shows only VGAT expression. E: Magnification of the double-labeled embryo in F showing VGAT-positive neurons in epifluorescence. F,H: Double-labeled 22-hr neurula. F: Magnification showing GAD and VGAT co-expression (dark brown) and VGAT expression (red). The arrow indicates the first pigment spot. G: Magnification of the bracketed region in F.