Figure 4

Fluorescent images of representative brains following injection with MMPSense750. Autofluorescence seen in the FITC channel (left hand column) is considered to indicate increased coenzyme levels such as NADH revealing the location of the stroke. A localized increase in intensity using the NIR channel (mid column) is observed following injection of MMPSense750, both acutely and subacutely following stroke but less appreciably in sham and hypothermia treated animals. These near infrared intensity changes correspond to MMP9 increases in immunohistochemical staining of coronal frozen sections shown from representative animals (right hand column).