Figure 7From: Selenium preserves mitochondrial function, stimulates mitochondrial biogenesis, and reduces infarct volume after focal cerebral ischemiaSelenium pretreatment alters autophagy activation following cerebral ischemia. A. Representative Western blot and analysis of autophagy markers Beclin 1 and LC3 shows that selenium pretreatment lowers that intensity Beclin 1 and LC3-II as compared to saline treatment. B. Immunolocalization of LC3 (green) and pDrp1 (red) indicates that cerebral ischemia leads to conversion of LC3-I (diffused) to LC3-II (punctated). Punctated LC3 pattern overlaps with pDrp1 staining. Selenium pretreatment, on contrary, decreases LC3-II (punctated) staining. The western results were normalized as relative % of control set at 100% (n = 4 each group). Data represents mean ± SD. *p < 0.05, and ***P < 0.001 vs control and ##p < 0.01, ###p < 0.001 vs. respective time point. Se = selenium and Cont = control. Bar = 100 μm.Back to article page