New highly sensitive rodent and human tests for soluble amyloid precursor protein alpha quantification: preclinical and clinical applications in Alzheimer’s disease

Table 4 Stability of h-sAPPα in CSF stored at different conditions

Effect of storage temperature
Samples	−80°C	−20°C	+4°C	RT	F/T
1	100 ± 6	101 ± 5	100 ± 11	93 ± 1	103 ± 2
2	100 ± 7	93 ± 9	95 ± 15	85 ± 14	93 ± 6
3	100 ± 1	77 ± 1	82 ± 4	80 ± 4	76 ± 4
4	100 ± 5	113 ± 2	80 ± 4	109 ± 3	99 ± 5
5	100 ± 3	86 ± 5	81 ± 4	70 ± 5	83 ± 1

Five CSF samples were divided in five aliquots and stored for 24 hours at one of several different temperatures including room temperature (RT), or stored at −80°C and exposed to three freeze/thaw cycles (F/T) within a period of three days.
The results were normalized (%) for the results from the aliquot stored at −80°C.

ISSN: 1471-2202