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Figure 5 | BMC Neuroscience

Figure 5

From: Expanded ataxin-7 cause toxicity by inducing ROS production from NADPH oxidase complexes in a stable inducible Spinocerebellar ataxia type 7 (SCA7) model

Figure 5

Reduced CAT levels, but increased levels of SOD1 and GSTA3 in ATXN7Q65-GFP expressing cells. A) Representative western blot analysis of GSTA3, SOD1 and CAT levels in FLQ65 cells induced to express ATXN7Q65-GFP for 0–12 days. B) Quantitative analysis of GSTA3 levels from three experiments as shown in A. C) Quantitative analysis of SOD1 levels from three experiments as shown in A. D) Quantitative analysis of CAT levels from three experiments as shown in A. E) NAC effect on the expression of GSTA3, SOD1 and CAT levels in ATXN7Q65-GFP expressing cells. FLQ65 cells not induced or induced to express ATXN7Q65-GFP for 9 days while growing in media with or without NAC (5 mM) were analyzed. F) Quantitative analysis of GSTA3 levels from three experiments with treatments as in E. G) Quantitative analysis of SOD1 levels from three experiments with treatments as in E. H) Quantitative analysis of CAT levels from three experiments with treatments as in E. All quantifications are shown as means ± SEM from three independent experiments with triplicates. * p <0.05, ** p <0.01 and *** p <0.001.

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BMC Neuroscience

ISSN: 1471-2202

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