Figure 1From: Methodology for extracting activity from functional calcium imaging dataSteps in extraction of activity episodes. Calculated frequency (34 kHz) is displayed in the figure heading. A. Sample trace (blue) and smoothed result of first Butterworth filter (red). B. Modified first derivative (black) and subsequent negated second derivative (blue). C. Identification of candidates that are local minima of second derivative (red, green) and final selected locations of activity episodes (green).Back to article page