Figure 5

Western blot analysis of the localization of MKP-7 in cytoplasm and nucleus after ischemia and the effect of inhibition of this MKP-7 translocation by LMB on JNK activity. LMB administered in rat hippocampus as described in the Methods, and its vehicle (Veh) was used as a control. Proteins were obtained from the cytoplasm and nucleus and measured using antibodies against p-JNK (Thr183/Tyr185) and MKP-7. A: MKP-7 levels in cytoplasm and nucleus after 4 or 24 h of reperfusion following 10 min of ischemia. B: Effect of LMB on JNK activity in cytoplasm and levels of MKP-7 in cytoplasm and nucleus after 4 h of reperfusion post-ischemia. OD data are presented as means ± SEM (n = 4) and expressed as magnitudes of the alteration versus sham control or control. *P < 0.05, versus sham control; ^#P < 0.05, comparison between chemically treated 4-h reperfusion groups and their vehicle control.