Figure 2From: Trophic and proliferative effects of Shh on motor neurons in embryonic spinal cord culture from wildtype and G93A SOD1 miceShh has trophic effects on motor neurons. Representative microscopic images show SMI32+ cells from WT mice treated with Shh 250 ng/ml had larger somata and more arborization (A) compared to cyclopamine-treated cells (B). It was shown that the average number of SMI32+ cells per field after treated with 250 ng/ml Shh was significantly higher than that control and cyclopamine (Shh 11 ± 1 vs control 5 ± 0, ** p = 0.0001; Shh 11 ± 1 vs cyclopamine 3 ± 0, * p = 0.0001; cyclopamine 3 ± 0 vs control 5 ± 0, δ p=0.017; C).Back to article page