Figure 3From: Wild-type but not mutant SOD1 transgenic astrocytes promote the efficient generation of motor neuron progenitors from mouse embryonic stem cellsExamination of the effect of astrocyte conditioned media on interneurons derived from differentiation of mouse embryonic stem cells. (a) Double-labelled fluorescence image of culture grown in wt non-Tg astrocyte CM labelled for Lhx1/5 interneurons (red), Hb9:eGFP MNs (green) and DAPI for nuclei (blue). Scale bar 50 μm. (b) Analysis of the number of interneurons in control medium, wt non-Tg and Tg SOD1G93A CM. Data gathered from 2 independent experiments, each condition examined in triplicate and analysed by one-way ANOVA followed by Tukey’s post-test. No significant differences in the proportion of interneurons were found between the three media.Back to article page