Glial phagocytosis of microspheres. Canine glia showed phagocytosis of microspheres. OB-OECs (A), OM-OECs (B) and SCs (C) contained 6 to 18 microspheres as indicated by confocal z-axis series analysis. XY, XZ, and YZ projections revealed that the microspheres were taken up and engulfed by the canine glia. OB-OECs, OM-OECs and Schwann cells incubated 2 h at 4°C showed no signs of engulfed microspheres indicating that phagocytosis is absent at low temperature (data not shown). Cells are stained against p75NTR (red) and DAPI (blue). YG microspheres appear cyan due to fluorescence in the blue and green spectrum. Scale bar is 10 μm.