Figure 5
From: Loss of IP3 receptor function in neuropeptide secreting neurons leads to obesity in adult Drosophila
Over-expression of itpr+in peptidergic neurons rescues feeding defect of itprkumutant. (A) Over-expression of itpr+ in peptidergic neurons rescues the feeding defects of itprku mutants as seen by the amount of red dye in their abdomen. (B) Spectrophotometric quantification of the red dye in fly abdomen. (C) Quantitative real-time PCR analysis of the transcript levels of gastric lipase CG5932 magro from gut tissue of peptidergic knock down of itpr is similar to that observed in itprku. Over-expression of itpr+ in the peptidergic neurons in the mutant background restores its levels to near wild type. *p < 0.05; Student’s t test; difference significant as compared to the wild type unless otherwise indicated.