Mn (III) tetrakis (4-benzoic acid) porphyrin scavenges reactive species, reduces oxidative stress, and improves functional recovery after experimental spinal cord injury in rats: comparison with methylprednisolone

Table 2 In vitro removal of O ₂ ^•- by MnTBAP and MPSS

Sample	Reagent added						Total volume	Final concentrations
	Cytochrome c (200 μM)	Xanthine oxidase	Xanthine (80 μM)	MnTBAP (80 mg/L)	MPSS (120 mg/L)	ACSF
		(100 unit/L)
Control	100 μl	100 μl	100 μl	0	0	100 μl	400 μl	50 μM cytochrome c, 25 unit/L xanthine oxidase, 20 μM xanthine
MnTBAP-treated	100 μl	100 μl	100 μl	100 μl	0	0	400 μl	50 μM cytochrome c, 25 unit/L xanthine oxidase, 20 μM xanthine, 20 mg/L MnTBAP
MPSS-treated	100 μl	100 μl	100 μl	0	100 μl	0	400 μl	50 μM cytochrome c, 25 unit/L xanthine oxidase, 20 μM xanthine, 30 mg/L MPSS