Figure 4

CD-45 immunoreactivity 3 or 7 days after a single injection in the CeA. A. 3 days after a single saline injection. B. 7 days after a single saline injection. C. 3 days after a single injection of AONs. D. 7 days after a single injection of AONs. E. CD-45 immunoreactivity in the CeA of an untreated mouse. F. Quantification of CD-45 immunoreactive area shown as percentage of the total area of visual field. One-way ANOVA followed by Tukey’s post hoc test revealed no significant differences between the respective AON and saline injected animals (one-way ANOVA F_(4,17)=1.092, p>0.39, N=3-7 animals per group). Quantification of IBA-1 immunoreactive area had similar results (one-way ANOVA F_(4,17)=1.535, p>0.23, data not shown). In conclusion, a single AON injection did not induce stronger microglia activation than saline. Scale bar 50 μm. Red: CD-45, blue: Hoechst. Green signal (AONs) has been omitted for clarity.