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Table 2 Antibodies and dilutions used for all immunofluorescent stainings

From: Antisense-mediated isoform switching of steroid receptor coactivator-1 in the central nucleus of the amygdala of the mouse brain

Primary antibodies

Secondary antibodies

Marker

Type

Manufacturer

Dilution

Type

Manufacturer

Dilution

CRH

Goat polyclonal

Santa Cruz biotechnology, Heidelberg, Germany

1:250

Donkey anti-goat

Invitrogen, Breda, the Netherlands

1:100

NeuN

Mouse monoclonal

Chemicon, Amsterdam, the Netherlands

1:200

Donkey anti-mouse

Invitrogen, Breda, the Netherlands

1:500

GFAP

Mouse monoclonal

Santa Cruz biotechnology, Heidelberg, Germany

1:1000

Donkey anti-mouse

Invitrogen, Breda, the Netherlands

1:500

IBA-1

Goat monoclonal

Santa Cruz biotechnology, Heidelberg, Germany

1:200

Donkey anti-goat

Invitrogen, Breda, the Netherlands

1:400

CD-45

Rat polyclonal

Serotec, Düsseldorf, Germany

1:1000

Donkey anti-rat

Invitrogen, Breda, the Netherlands

1:500

  1. The fluorophore of all secondary antibodies was Alexa Fluor® 594.