Primary antibodies | Secondary antibodies | |||||
---|---|---|---|---|---|---|
Marker | Type | Manufacturer | Dilution | Type | Manufacturer | Dilution |
CRH | Goat polyclonal | Santa Cruz biotechnology, Heidelberg, Germany | 1:250 | Donkey anti-goat | Invitrogen, Breda, the Netherlands | 1:100 |
NeuN | Mouse monoclonal | Chemicon, Amsterdam, the Netherlands | 1:200 | Donkey anti-mouse | Invitrogen, Breda, the Netherlands | 1:500 |
GFAP | Mouse monoclonal | Santa Cruz biotechnology, Heidelberg, Germany | 1:1000 | Donkey anti-mouse | Invitrogen, Breda, the Netherlands | 1:500 |
IBA-1 | Goat monoclonal | Santa Cruz biotechnology, Heidelberg, Germany | 1:200 | Donkey anti-goat | Invitrogen, Breda, the Netherlands | 1:400 |
CD-45 | Rat polyclonal | Serotec, Düsseldorf, Germany | 1:1000 | Donkey anti-rat | Invitrogen, Breda, the Netherlands | 1:500 |