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Table 2 Mating, copulation duration and fertility phenotypes of FRU M -depleted males

From: A small cohort of FRUM and Engrailed-expressing neurons mediate successful copulation in Drosophila melanogaster

Male genotype

Percent mated (%)

Mean copulation duration ± SEM

Percent fertile (%)

WT

67 (n = 19)

15.6 ±1.0 (n = 19)

88 (n = 16)

En-GAL4/+

85 (n = 20)

15.3 ± 0.53 (n = 20)

84 (n = 16)

fruMRNAi/+, UAS-GAL4-I /+

95 (n = 20)

16.7 ± 1.0 (n = 20)

90 (n = 20)

En-fru M RNAi

57 (n = 28)

10.9 ± 1.5 (n = 13)

61 (n = 23)

En-fruMRNAi, UAS-GAL4-I

19 (n = 21)

11.3 ± 4.4 (n = 21)

25 (n = 20)

En-fruMRNAi, UAS-GAL4-II

35 (n = 20)

12.9 ± 2.2 (n = 20)

40 (n = 20)

En-fruMRNAi, UAS-GAL4-III

55 (n = 20)

13.0 ±1.6 (n = 20)

67 (n = 21)

En-fruMRNAi, Cha-GAL80

30 (n = 20)

19.0 ± 3.9 (n = 20)

42 (n = 19)

  1. All males were raised and maintained at 29°C. Single males (n = number tested) and females were paired for 30 minutes and the percent mated and the mean copulation duration calculated in minutes. Fertility tests were carried out at 29°C between an individual male and 2–3 virgin WT females for 7 days and the presence of larvae determined. For these experiments, the En-GAL4 chromosome had been backcrossed into a w- background for four generations.