Figure 8

Characterisation of optimised RBEC and RSCEC barriers for use in small molecules drug studies. Intracellular accumulation of the fluorescent P-gp efflux transporter substrate rhodamine 123 in (a) RBECs (n = 4) and (b) RSCECs (n = 3) cultured on cell culture inserts in the absence and presence of the inhibitor verapamil. Data is presented as mean ± SEM and was analysed using an unpaired, two-tailed students t-test, *P < 0.01, **P < 0.001. Permeability coefficients for CNS-permeable and impermeable small molecule drugs were calculated across (c) RBEC (n = 5 independent cell culture experiments) and (d) RSCEC monolayers cultured on cell culture inserts (n = 3 independent cell culture experiments).