Table 1 List of antibodies and lectins used for immunolabeling
Antigen | Immunogen | Host | Dilution | Source |
|---|---|---|---|---|
Calbindin | Purified Calbindin from bovine kidney | mouse | 1:300 | Sigma, St Louis, MO (Catalog # C9848; clone CB-955) |
GAD-65 | Immunoaffinity purified GAD from rat brain | mouse | 1:500 | Developmental Studies Hybridoma Bank, U Iowa, (Catalog # GAD-6; clone GAD-6) |
GlyT1 | C-terminus peptide sequence from Rat GlyT1 | goat | 1:500 | Chemicon, Temecula, CA (Catalog # AB1770) |
MAP-1 | Microtubules purified from rat brain | mouse | 1:300 | Sigma, St Louis, MO (Catalog # M4278; clone HM-1) |
PKC | Human PKCα, aa. 270-427 | mouse | 1:100 | B-D Transduction Labs, San Jose, CA (Catalog # 610107; clone 3/PKC) |
PNA | — — | — — | 1:20 | Invitrogen-Molecular Probes, Carlsbad, CA (Catalog # L21409) |
P-Rex2 | Mouse P-Rex2, aa. 717-799 | rabbit | 1:1000 | Dr. Heidi C.E. Welch, The Babraham Institute, Cambridge, England (affinity purified antiserum #78) |
Synapto-tagmin 2 | Zebrafish hindbrain membranes | mouse | 1:200 | Zebrafish International Resource Center, Eugene, OR (Catalog # znp-1; clone znp-1) |
Syntaxin 1 | Rat hippocampus synaptosomes | mouse | 1:500 | Sigma, St Louis, MO (Catalog # S0664; clone HPC-1) |
VGluT1 | Peptide, aa. 541–560 of rat VGluT1 | guinea pig | 1:1000–1:2000 | Chemicon, Temecula, CA (Catalog # AB5905) |
VGluT1 | Peptide, aa. 493–560 of rat VGluT1 | mouse | 1:100–1:300 | UC Davis/NIH NeuroMab Facility, Davis, CA (Catalog # 75–066; clone N28/9) |