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Table 1 List of antibodies and lectins used for immunolabeling

From: P-Rex2, a Rac-guanine nucleotide exchange factor, is expressed selectively in ribbon synaptic terminals of the mouse retina

Antigen Immunogen Host Dilution Source
Calbindin Purified Calbindin from bovine kidney mouse 1:300 Sigma, St Louis, MO (Catalog # C9848; clone CB-955)
GAD-65 Immunoaffinity purified GAD from rat brain mouse 1:500 Developmental Studies Hybridoma Bank, U Iowa, (Catalog # GAD-6; clone GAD-6)
GlyT1 C-terminus peptide sequence from Rat GlyT1 goat 1:500 Chemicon, Temecula, CA (Catalog # AB1770)
MAP-1 Microtubules purified from rat brain mouse 1:300 Sigma, St Louis, MO (Catalog # M4278; clone HM-1)
PKC Human PKCα, aa. 270-427 mouse 1:100 B-D Transduction Labs, San Jose, CA (Catalog # 610107; clone 3/PKC)
PNA — — — — 1:20 Invitrogen-Molecular Probes, Carlsbad, CA (Catalog # L21409)
P-Rex2 Mouse P-Rex2, aa. 717-799 rabbit 1:1000 Dr. Heidi C.E. Welch, The Babraham Institute, Cambridge, England (affinity purified antiserum #78)
Synapto-tagmin 2 Zebrafish hindbrain membranes mouse 1:200 Zebrafish International Resource Center, Eugene, OR (Catalog # znp-1; clone znp-1)
Syntaxin 1 Rat hippocampus synaptosomes mouse 1:500 Sigma, St Louis, MO (Catalog # S0664; clone HPC-1)
VGluT1 Peptide, aa. 541–560 of rat VGluT1 guinea pig 1:1000–1:2000 Chemicon, Temecula, CA (Catalog # AB5905)
VGluT1 Peptide, aa. 493–560 of rat VGluT1 mouse 1:100–1:300 UC Davis/NIH NeuroMab Facility, Davis, CA (Catalog # 75–066; clone N28/9)