Figure 4

MEK and PKA inhibition reduces the up-regulation of BDNF induced by DOM insult. Organotypic slices were cultured in the presence of the MEK inhibitor PD98059 (A), the PKA inhibitor H89 (B) or the CaMKII inhibitor KN93 (C) for 1 hour and then treated with 2 μM DOM for 24 h (insult). Lysates were collected immediately after the 24 h DOM-treatment (0 hours post-insult, HPI) or after 24 h resting in the DOM-free medium (24 HPI), analyzed by immunobloting using a BDNF antibody and the membranes were reprobed with β-actin as a loading control. The blots correspond to representative experiments and values are the means ± SEM of at least three experiments performed from different cultures (#P < 0.01 vs. – PD98059, H89 or KN93 within the same group; *P < 0.01 vs - PD98059, H89 or KN93 within C group).