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Figure 2 | BMC Neuroscience

Figure 2

From: Steerable-filter based quantification of axonal populations at the developing optic chiasm reveal significant defects in Slit2−/− as well as Slit1−/−Slit2−/−embryos

Figure 2

Combining data from multiple embryos with different Slit1 and Slit2 genotypes. (A-D) Images depict examples of chiasms analyzed using the steerable filter algorithm, with conventions as in Figure 1E. Scale bars = 200 μm. (E-H) Plots show the direction vector fields overlaid (using the grid system in Figure 1A) from all embryos of each genotype, with each colour representing data from a different embryo. Only small subsets of arrows from each embryo have been plotted, for clarity. (I-L) Heat-maps: In each square we calculated the mean number of arrows, including all arrows from all embryos of each genotype (these values for mean densities of arrows are proportional to the mean densities of axon). A colour scale is used to illustrate the spatial distribution of mean densities on a relative scale for each genotype. A, E, I, wild-type, n = 9; B, F, J, Slit1−/−, n = 11; C,G,K, Slit2−/−, n = 5; D,H,L, Slit1−/−Slit2−/−, n = 4.

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