Figure 3

Areas of statistically significant differences between wild-type and mutant chiasms. In each panel the white squares indicate positions that differ significantly from wild-type; Student’s t-test was applied for axon number and Watson-Williams test for orientation and curvature. All tests were controlled for false positives [14]; α = 0.05; see Materials and Methods). Columns show differences in mean axon number, axon orientation, angle of axon curvature and magnitude of axon curvature per grid square. (A-D) Slit1^−/− embryos; (E-H) Slit2^−/− embryos; (I-L) Slit1^−/−Slit2^−/− embryos. Wild-type, n = 9; Slit1^−/−, n = 11; Slit2^−/−, n = 5; Slit1^−/−Slit2^−/−, n = 4.