Figure 3From: Growth hormone pathways signaling for cell proliferation and survival in hippocampal neural precursors from postnatal miceGH treatment prevents apoptosis in SGZ neurospheres. A) Neurospheres growing in defined media were placed in defined media without EGF and FGF2, and 24 h later treated with GH (500 ng/mL), pegvisomant (20 μg/mL), or GH + pegvisomant for an additional 48 h period. Control cells were treated with saline. Cell apoptosis was determined by TUNEL staining. Each bar represents the mean + SEM of 3 experiments in triplicate. * = p <0.05 vs Control; ° = p <0.05 vs Control and p <0.01 vs GH. Basal apoptosis was determined by growing the cells in the defined media (Control + EGF + FGF2). Apoptosis values in this group were significantly lower (p <0.01) than in the other groups. E = EGF; F = FGF2. B) Representative images of results presented in A. Scale bar = 10 μm.Back to article page