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Figure 3 | BMC Neuroscience

Figure 3

From: Hypocretin1/orexinA-immunoreactive axons form few synaptic contacts on rat ventral tegmental area neurons that project to the medial prefrontal cortex

Figure 3

Confocal photomicrographs showing Fluorogold (FG)- and/or tyrosine hydroxylase (TH)-labeled neurons in the ventral tegmental area (VTA) of an animal with an FG injection in the prelimbic cortex. (A-C) , Biomappings showing green FG-labeling (A), red TH-labeling (B) and both (FG/TH) labelings (orange) in the merged image (C). (D-F) , High power confocal photomicrographs of the boxed area in A corresponding to the VTA ipsilateral to the FG injection, depicting FG (D), TH (E) and their merged images (F). Arrowhead marks one VTA neuron dually labeled for FG and TH. Scale bar, A-C, 400 μm; D-F, 40 μm.

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