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Table 1 Fluorogold-labeled neurons in the ventral tegmental area by either immunofluorescence or immunohistochemistry

From: Hypocretin1/orexinA-immunoreactive axons form few synaptic contacts on rat ventral tegmental area neurons that project to the medial prefrontal cortex

  

PL (%)

PL-MO (%)

Cg1-PL (%)

IH

Ipsilateral

84.18 ± 2.11

93.63 ± 2.61

88.06 ± 1,11

contralateral

15.82 ± 2.11

6.37 ± 2.61

11.94 ± 1.11

IF

Ipsilateral

85.60 ± 4.77

90.47 ± 3.17

85.56 ± 6.93

contralateral

14.40 ± 4.77

9.53 ± 3.17

14.44 ± 6.93

  1. Percentage of Fluorogold (FG)-immunoreactive neurons (Mean ± SE) within the ipsilateral and the contralateral ventral tegmental area to the FG injection site in the three injection site groups using immunohistochemistry (IH) or immunofluorescence (IF) techniques. Cg1: cingular cortex (n = 4 animals); MO: medial orbital cortex (n = 4 animals); PL: prelimbic cortex (n = 10 animals). Percentages were calculated from the FG-immunolabeled neurons counted in 8 vibratome sections per animal in IH experiments and in 5 vibratome sections per animal in IF experiments.