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Figure 6 | BMC Neuroscience

Figure 6

From: Microparticles in multiple sclerosis and clinically isolated syndrome: effect on endothelial barrier function

Figure 6

Effect of MPs on hCMEC/D3 barrier function. (A) Percentage of TEER decrease after 14 hours of incubation with the indicated MPs at 400 and 2000 MP/μl. Left graph, control donors. Right graph, RRMS patients (RR). Average TEER decrease in response to TNF is marked by discontinuous lines. Bottom table shows the percentage of MPs inducing a response stronger than control TNF on the endothelial barrier. *p = 0.04. (B) VE-cadherin, ZO-1 and F-actin staining in HCMEC/D3 cells exposed for 14 h to MPs from a donor and a RRMS patient. MPs did not induced big gaps in HCMEC/D3, as in HUVECs, but, instead, RR-MPs dispersed the junctional staining of VE-cadherin (top right graph) and ZO-1 (bottom right graph) quantified as the ratio between the staining intensity at cell-cell borders and at the inner cell area (Junctional index). **p < 0.03, ***p < 0.002. Bar, 20 μm.

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