Figure 3From: ROS-mediated activation of Drosophila larval nociceptor neurons by UVC irradiationIncreased ROS levels in response to UVC irradiation. Confocal microscope images of w1118 dissected larval body walls that were incubated with 10 μM carboxyl-H2DCFDA prior to UVC irradiation. (AB) Larval body wall images at epidermal focal plane. (A) Control wild-type (w[1118]) larvae showed no detectable fluorescence in the absence of UVC irradiation. (B) UVC-irradiated larval body walls displayed a significant increase in fluorescence in epidermal tissues indicating production of ROS in response to irradiation. (CD) Larval body wall images at muscle layer focal plane. (C) Wild-type controls showed no detectable fluorescence in the absence of UVC irradiation. (D) UVC-irradiated larval body walls displayed a significant increase in fluorescence in muscle layers indicating production of ROS in response to irradiation.Back to article page