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Table 2 Effect of soman (105 μg/kg) on c-Fos and Bax mRNA levels in different brain regions

From: Efficacy of antidotes (midazolam, atropine and HI-6) on nerve agent induced molecular and neuropathological changes

Time points

Fold change over control

Hippocampus

Cerebral cortex

Cerebellum

Gene name

c-Fos

Bax

c-Fos

Bax

c-Fos

Bax

Control animals

1.0±0.18

1.0±0.21

1.0±0.24

1.0 ±0.18

1.0 ±0.16

1.0±0.13

Soman exposed

      

30 min

3.5*±0.47

2.6*±0.13

5.0* ±0.8

2.5*±0.24

3.0*±0.4

0.72±0.24

2.5 h

4.2*±0.18

1.6*±0.31

3.1*±0.71

2.0*±0.3

1.8*±0.25

0.75±0.14

1 day

3.4*±0.4

1.8*±0.27

3.8*±0.8

1.6*±0.18

1.5*±0.19

0.45*±0.18

7 day

0.65±0.3

1.5*±0.18

0.78±0.23

1.25±0.25

0.8±0.18

0.52*±0.09

  1. Quantitative real-time RT-PCR was carried out and results are presented as fold change over control (presented as 1.0 fold) after normalizing the data (n=4 per each time point) with housekeeping gene, RNA polymerase-II (RP-II), which did not show any significant changes in the mRNA expression among control and experimental groups (Data not presented). *Significantly different from control group. A difference of p< 0.05 was considered significant.