Dynamic activation and proliferation of GFAP+ astrocytes after PT. A) Representative fluorescent images of GFAP and Brdu double stained brain sections from mice at different times after PT. Right panels are the high resolution confocal images of boxed regions in the left panels. B) The density of GFAP+ cells in the penumbra. C-D) The density and cumulative density of GFAP+Brdu+ cells in the penumbra. E) The percentage of GFAP+Brdu+ double stained cells out of total Brdu+ cells in the penumbra. F) The percentage of GFAP+Brdu+ double stained cells out of total GFAP+ cells in the penumbra. In Figure (E-F), the cells were counted in the penumbral region of 200 μm × 400 μm in layers 2/3 cortex located 0-400 μm from the edge of ischemic core. N=5 mice in each time point. *p<0.05, compared with value at day 4 after ischemia using ANOVA test.