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Figure 4 | BMC Neuroscience

Figure 4

From: Defined α-synuclein prion-like molecular assemblies spreading in cell culture

Figure 4

Thioflavin-S (ThS) positive staining of endogenous α-syn aggregates. SH-SY5Y cells were infected with recombinant human α-syn short amyloid fibrils (SF: short amyloid fibrils of α-syn) and sub-passed at sixth passage (p6). The deposition and level of α-syn (red) in α-syn-infected cell lines after six passages were detected by anti-α-syn antibody (Additional file 6: Table S2). The colocalization was observed between the ThS signal (yellow) and anti-human α-syn antibody (red). The nuclei (blue) were stained with DAPI. Scale bars, 12 μm.

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