Figure 6

FISH detection of GP and GS mRNA in cultured trigeminal neurons. a-c ICC for GP combined with β-tubulin ICC. GP protein is present in neuronal cell bodies and processes (arrows in a-c point to a long neuronal process expressing GP). d-f FISH for GP combined with MAP 2 ICC, culture derived from postnatal trigeminal ganglia, 3 DIV. GP mRNA is abundant in neuronal cell bodies but absent from MAP 2 positive processes. g-i Same as in d-f, but cultures derived from embryonic ganglia, 2 DIV. GP mRNA is only present in neuronal cell bodies. j-l Combination of GS FISH with MAP 2 ICC, cultures derived from PD 2 ganglia. GS mRNA is only present in cell bodies and nuclear speckles (j). Bar in a = 10 μm and applies to a-c, bar in d = 10 μm and applies to d-f, bar in g = 20 μm and applies to g-i, bar in j = 20 μm and applies to j-l.