Figure 5
From: BAC-based cellular model for screening regulators of BDNF gene transcription
Treatment with ionomycin induces reporter expression in different rBDNF-hRluc-EGFP cell lines. (A) Luciferase activity in 1 μM ionomycin treated cells relative to vehicle (0.1% DMSO) treated cells (dashed line) at each time point, measured in live cells during 12 hours. Renilla luciferase luminescence was measured using Enduren substrate 2–12 hours after the start of the treatment at one hour intervals. Error bars indicate SD of three independent experiments. (B) Cell viability in ionomycin treated cells relative to vehicle treated cells after 12 hours in different cell lines. Viability was assayed by cellular ATP levels. Error bars indicate SD of three independent experiments.