Effects of SPS on the intensity and localization of MR- and GR-ir in the amygdala. Dual-immunofluorescent images for the MR- and GR-ir in the amygdala. Images of MR-ir (red; left panel), GR-ir (green; middle panel), and colocalization of MR- and GR-ir (yellow; right panel) in the control group (A), 1 day SPS (B), 7 days SPS (C), and 14 days SPS (D). E shows quantitative analysis of MR and GR intensity using immunohistochemistry. The intensity of MR- and GR-ir decreased in the 1 day SPS, and remained virtually unchanged in the 7 days, 14 day SPS in comparison with control group. MR- (arrow) and GR-ir (arrow head) showed more cytoplasmic distribution in the amygdala of the 7 days and 14 days SPS. *P < 0.05 vs. the control group.