Simultaneous monitoring of cerebral metal accumulation in an experimental model of Wilson’s disease by laser ablation inductively coupled plasma mass spectrometry

Table 2 Average concentration of Fe, Cu, Zn and Mn in small hippocampal regions of the brain

μg g⁻¹wet weight	Control	WD	% change	p- value
Fascia dentata, layers
Moleculare
Fe	13.1 ± 2.1	14.6 ± 1.2		.1
Cu	5.2 ± 1.2	8.9 ± 1.2	+71%	.00001
Zn	14.0 ± 3.7	19.4 ± 1.3	+38%	.004
Mn	0.34 ± 0.10	0.32 ± 0.07		.9
Granulare
Fe	18.4 ± 3.4	16.3 ± 2.1		.2
Cu	4.1 ± 1.1	7.6 ± 1.0	+86%	.00001
Zn	25 ± 7	32 ± 3	+30%	.02
Mn	0.39 ± 0.14	0.39 ± 0.09		.7
Multiforme /polymorph
Fe	12.9 ± 1.7	13.2 ± 0.8		.7
Cu	4.1 ± 1.2	8.0 ± 1.5	+95%	.0001
Zn	47 ± 14	59 ± 9	+24%	.1
Mn	0.48 ± 0.28	0.48 ± 0.14		.8
Cornu ammonis, layers
Oriens
Fe	10.8 ± 1.2	10.6 ± 1.0		.7
Cu	3.8 ± 0.8	6.6 ± 1.4	+75%	.0002
Zn	18.0 ± 4.0	20.8 ± 3.8		.2
Mn	0.26 ± 0.06	.32 ± 0.08		.04
Pyramidale, CA3
Fe	11.3 ± 1.9	12.3 ± 1.4		.3
Cu	4.6 ± 1.4	7.0 ± 1.2	+52%	.003
Zn	33 ± 10	38 ± 5.5		.2
Mn	0.45 ± 0.20	0.48 ± 0.13		.5
Pyramidale, CA1-2
Fe	19.0 ± 3.7	15.1 ± 1.5		.03
Cu	3.5 ± 1.0	5.9 ± 0.9	+69%	.0002
Zn	20.9 ± 4.8	24.4 ± 3.4		.1
Mn	0.27 ± 0.07	0.33 ± 0.06		.04
Radiatum
Fe	10.5 ± 1.5	10.2 ± 0.7		.7
Cu	3.2 ± 0.8	6.3 ± 1.2	+92%	.00002
Zn	21.8 ± 4.9	23.8 ± 3.0		.3
Mn	0.25 ± 0.06	0.31 ± 0.08		.04
Lacunosum- moleculare
Fe	12.8 ± 2.1	12.2 ± 1.3		.6
Cu	3.6 ± 0.9	5.6 ± 1.0	+53%	.001
Zn	14.1 ± 3.7	18.4 ± 1.5	+30%	.01
Mn	0.28 ± 0.08	0.29 ± 0.06		.4
Subiculum
Fe	14.4 ± 2.3	12.9 ± 1.4		.2
Cu	3.4 ± 0.9	5.9 ± 1.1	+72%	.0001
Zn	15.4 ± 4.1	17.4 ± 1.6		.2
Mn	0.23 ± 0.06	0.27 ± 0.03		.7
Praesubiculum
Fe	13.9 ± 3.2	13.8 ± 1.5		1
Cu	4.8 ± 1.4	7.8 ± 1.4	+62%	.0006
Zn	13.1 ± 3.0	16.3 ± 1.0	+25%	.02
Mn	0.23 ± 0.07	0.26 ± 0.05		.2

Concentrations of Fe, Cu, Zn, Mn (mean ± SD) measured by LA-ICP-MSI in subregions of the hippocampal formation. Layers are given in the order from outside to inside.

ISSN: 1471-2202