Figure 6

Western analysis of frontal cortex CALB abundance in adult male rats exposed to either the Phyto-600 or Phyto-free diets. A. Densiometric analysis of CALB frontal cortex Western autoradiograms. B. Abundance of cytochromeoxidase subunit 4 served as controls (for loading). C. Histogram of CALB abundance by treatment. For each immunoblot, the cytochromeoxidase band was divided into the CALB band within each lane, then the lowest intensity band was assigned an arbitrary number (1) and all other bands were expressed as a fraction of this value. The immunoblots analyzed represent the Mean ± SEM, averaging the band values from 4 to 6 independent blots). ^* Calbindin levels are significantly decreased in Phyto-600 males compared to Phyto-free male values.