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Table 2 Agrin and LN1 aggregation stimulus protocols. Note that the medium for all cultures was changed at t = 24 hrs, either to fresh medium or medium with stimulus.

From: Mechanistic distinctions between agrin and laminin-1 induced aggregation of acetylcholine receptors

Treatment

Abbreviation

Stimulus

1) Control

C

None

2) Agrin alone

A

Agrin 24–48 hrs

3) LN1 alone

L

LN1 24–48 hrs

4) Agrin + LN1

AL

Agrin+LN1 24–48 hrs

5) Agrin then LN1

A>L

Agrin 24–26 hrs: LN1 26–48 hrs

6) LN1 then Agrin

L>A

LN1, 24–26 hrs: Agrin 26–48 hrs