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Figure 2 | BMC Neuroscience

Figure 2

From: The predominantly HEAT-like motif structure of huntingtin and its association and coincident nuclear entry with dorsal, an NF-kB/Rel/dorsal family transcription factor

Figure 2

LPS signal-dependent, concomitant nuclear accumulation of the prenylated carboxyl-terminal fragment of Drosophila huntingtin and dorsal. a, Dhd2194C-myc-CKML has a prenylation-mediated membrane-targeting motif, Cys-Lys-Met-Leu sequence, derived from Drosophila ras protein, at the end of Dhd2194C-myc. Co-transfection of pIZ-Dhd2194C-myc-CKML and pIZ-NLS-GFP-lacZ showed cytoplasmic distribution of Dhd2194C-myc-CKML. b, Co-expression of Dhd2194C-myc-CKML and FLAG-Dorsal causes dot-like staining without apparent nuclear accumulation of both proteins. c, LPS stimulation to the cells expressing Dhd2194C-myc-CKML and FLAG-Dorsal caused nuclear accumulation of both proteins. The same phenomena were observed using another prenylation motif construct, pIZ-Dhd2194C-myc-CAAC (data not shown).

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BMC Neuroscience

ISSN: 1471-2202

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