Figure 2
From: Insulin signaling inhibits the 5-HT2Creceptor in choroid plexus via MAP kinase
Effect of insulin and IGF-1 on the peak Cl- current in response to serotonin or acetylcholine in Xenopus oocytes expressing 5-HT 2C and M1 acetylcholine receptors. (A) Effects of insulin or IGF-1 on the peak Cl- current in response to 5-HT. Top left, representative Cl- current trace after stimulation with 5-HT. Top right, representative Cl- current trace after stimulation with 5-HT in an oocyte pre-treated with insulin. Horizontal bars above the current traces indicate bath application of 1 μM 5-HT. Middle, effects of insulin on the peak Cl- current induced by 5-HT. Bottom, effects of IGF-1 on the peak Cl- current induced by 5-HT. (B) Effects of insulin or IGF-1 on the peak Cl- current in response to Ach. Top left, representative current trace after stimulation with Ach. Top right, representative current trace after stimulation with Ach in an oocyte previously treated with insulin. Horizontal bars above the current traces indicate bath application of 10 μM Ach. Middle, effects of insulin on the peak Cl- current induced by Ach. Bottom, effects of IGF-1 on the peak Cl- current induced by Ach. The data are presented as mean ± S.E.M. of 12-16 oocytes (*, P < 0.05).