Figure 4

Identification of Interposed HindIII Fragment by Long Range PCR and Restriction Enzyme Digestion of HindIII Subclone of Genomic Clone c241. PCR was carried out using the zebrafish BDNF genomic clone c241 as a template. The primers were ex1aP3 and sc1P18. An aliquot of the PCR products (lane 1) was digested with HindIII (lane 2). A HindIII subclone of genomic clone c241 was also digested with HindIII. The PCR and digestion products were separated by electrophoresis on a 1.2% agarose gel containing ethidium bromide. Lane 1: PCR product. Lane 2: HindIII digest of PCR product. Lane 3: HindIII digest of subclone. Lane 4: HindIII digest of phage lambda. Sizes of fragments are shown in the right hand margin.