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Table 1 Characteristics of IBa via CaV2.1/β4 or CaV2.1/β4/α2δ2 with or without γ2 or γ4

From: Human neuronal stargazin-like proteins, γ2, γ3 and γ4; an investigation of their specific localization in human brain and their influence on CaV2.1 voltage-dependent calcium channels expressed in Xenopus oocytes.

Channel

Activation

 

n

 

V0.5act(mV)

kact.

Gmax (μS)

Peak at +10 mV (μA)

 

CaV2.1/β4

-2.60 ± 0.65

5.39 ± 0.49

8.45 ± 2.20

-0.25 ± 0.05

16

CaV2.1/β42

-4.27 ± 1.24

4.91 ± 0.52

9.96 ± 3.34

-0.32 ± 0.12

14

CaV2.1/β44

-5.70 ± 2.26

5.43 ± 0.47

8.16 ± 2.59

-0.26 ± 0.07

11

CaV2.1/β4/α2δ2

-4.60 ± 0.59

4.03 ± 0.15

14.97 ± 2.05 *

-0.54 ± 0.08

43

CaV2.1/β4/α2δ22

-4.13 ± 0.63

4.46 ± 0.14

15.43 ± 1.96

-0.54 ± 0.07

34

CaV2.1/β4/α2δ24

-5.56 ± 0.60

3.96 ± 0.24

14.8 ± 2.64

-0.56 ± 0.10

20

  1. To analyze the voltage-dependent activation, current-voltage (I-V) relationships (Figure 7A and Figure 7B) were fitted with a modified Boltzmann equation (see methods) to calculate V50act, the mid-point of voltage dependence of activation, kact, the slopefactor and Gmax, the maximum conductance. Values are expressed as mean ± S. E. M. of the number of replicates, n. One-way analysis of variance (ANOVA) tests determined no statistically significant changes in any pairs of data differing by the presence of a γ2 or γ4 P < 0.05 was considered significant). Statistically significant differences observed between the CaV2.1/β4 and CaV2.1/β4/α2δ2 channels are indicated by * (P < 0.05).