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Figure 1 | BMC Neuroscience

Figure 1

From: Equine estrogens differentially inhibit DNA fragmentation induced by glutamate in neuronal cells by modulation of regulatory proteins involved in programmed cell death

Figure 1

Detection of glutamate (5 to 20 mM) induced DNA fragmentation (DNA ladder) and its prevention by some equine (10 μM) estrogens. A: Lane: 1 = 1 kb DNA ladder; 2 = control HT22 DNA; 3 = 5 mM glutamate; 4 = 10 mM glutamate; 5 = 20 mM glutamate; 6 = 10 mM glutamate (18 h). B: HT22 cells treated with 10 mM glutamate for 18 h in presence or absence of estrogens. Lane: 1 = 17α-E2; 2 = control; 3 = 10 mM glutamate; 4 = 17β-E2; 5 = Eq; 6 = 17β-Eqn; 7 = 17α-Eqn; 8 = Δ8-E1. C: Lane: 1 = 1 kb DNA ladder; 2 = control; 3 = 10 mM glutamate alone; 4 = E1; 5 = Eqn; 6 = 17β-Eq; 7 = 17α-Eq; 8 = Δ8,17β-E2. Gel electrophoresis was carried out as described under methods and the gels were stained with ethidium bromide.

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