Figure 1From: Equine estrogens differentially inhibit DNA fragmentation induced by glutamate in neuronal cells by modulation of regulatory proteins involved in programmed cell deathDetection of glutamate (5 to 20 mM) induced DNA fragmentation (DNA ladder) and its prevention by some equine (10 μM) estrogens. A: Lane: 1 = 1 kb DNA ladder; 2 = control HT22 DNA; 3 = 5 mM glutamate; 4 = 10 mM glutamate; 5 = 20 mM glutamate; 6 = 10 mM glutamate (18 h). B: HT22 cells treated with 10 mM glutamate for 18 h in presence or absence of estrogens. Lane: 1 = 17α-E2; 2 = control; 3 = 10 mM glutamate; 4 = 17β-E2; 5 = Eq; 6 = 17β-Eqn; 7 = 17α-Eqn; 8 = Δ8-E1. C: Lane: 1 = 1 kb DNA ladder; 2 = control; 3 = 10 mM glutamate alone; 4 = E1; 5 = Eqn; 6 = 17β-Eq; 7 = 17α-Eq; 8 = Δ8,17β-E2. Gel electrophoresis was carried out as described under methods and the gels were stained with ethidium bromide.Back to article page