Figure 1

Sap47 gene structure and protein isoforms. (A). Top: Exon-intron structure of the Sap47 gene of Drosophila melanogaster. Boxes represent exons 1 to 11, coding sequences are black. I-V: Five proposed splice isoforms of the Sap47 gene. The corresponding calculated molecular masses are given on the right. (B) Western blot analysis of head homogenates from wild type (WT, w¹¹¹⁸) and Sap47 null mutant flies (control for specificity of signals). On the original blot 9 SAP47 bands can be identified as indicated on the left. Staining of cysteine string proteins (CSP) with monoclonal antibody ab49 serves as loading control.