Figure 4

Structure of the two different RNAi constructs. (A). Schematic of the Sap47 cDNA RNAi construct regulated by the yeast UAS enhancer. The transgene produces dsRNA as the construct carries the same fragment of the Sap47-I cDNA arranged as a dimer with dyad symmetry without a heterologous spacer. (B). Genomic-cDNA RNAi construct for the Sap47 gene. Exons 3 to 7 and part of exon 8 with their intervening sequences (introns 3 to 7) of the Sap47 gene were fused to an inverted corresponding fragment of the Sap47-I cDNA and cloned into the pUAST plasmid.