Figure 2

Reduced BrdU labeling in R6/2 mice, but no reversal by asialoEPO. All mice were injected with 50 mg/kg of BrdU i.p. every 12 h for their last six days of survival in order to assess whether asialoEPO would affect cell proliferation in the R6/2 mice at the age of 12 weeks. The number of BrdU labeled cells was assessed by stereology in the subventricular zone in five striatal sections/mouse. In this region, there was only a trend towards a reduced number of BrdU labeled cells in R6/2 mice compared to their wild-type littermates (A; one factor ANOVA, F (2, 16) = 1.63, n.s.). In the dentate gyrus, BrdU labeled cells were assessed in 7–9 sections/mouse from the first anterior aspect of the structure, and the number was expressed as the average number per section (B). The data were analyzed using a one factor ANOVA (F (2, 16) = 23.50, p < 0.0001) that revealed a significant difference between the groups. In this region, we found that there was a reduced number of BrdU labeled cells in R6/2 mice compared to wild-type mice (Bonferroni-Dunn post-hoc test, p < 0.0001). However, the administration of asialoEPO was not able to reverse the reduced cell proliferation (Bonferroni-Dunn post-hoc test, p > 0.05).