Phase contrast images illustrate RPE with pigment granules aggregated (left) and RPE with pigment granules dispersed (right). RPE were isolated from bluegill and treated with 10 μM forskolin for 45 minutes to induce aggregation. A subset was fixed overnight and prepared for microscopy. In a second subset forskolin was washed out, and RPE were treated with 100 nM carbachol to induce dispersion. After 45 minutes, the second subset was fixed overnight and prepared for microscopy. Images were obtained using a digital camera focused through the oculars of a Zeiss microscope. Scale bars = 10 μm.