Figure 3

Non-phosphorylatable triple-mutant Foxo3a triggers motoneuron death. (A) Purified motoneurons were coelectroporated with constructs for constitutively active (ca) Akt or empty vector, together with another vector encoding GFP. The survival of transduced motoneurons was evaluated after 2 d in culture with NTFs. Akt ca further enhances the effects of NTFs on motoneuron survival. Results are mean ± SD of 2 independent experiments. (B) Using the same protocol, motoneurons were electroporated with vectors encoding either HA-wt-Foxo3a or HA-TM-Foxo3a, together with the GFP vector. They were cultured in the presence or the absence of NTFs and survival was evaluated 2 d later. Overexpression of TM-Foxo-3a leads to reduced survival in each condition. Results are mean ± SD of 12 wells from 6 independent experiments. The 100 % corresponds to the number of motoneurons electroporated with HA-wt-Foxo3a. Differences between overexpression of HA-TM-Foxo3a and HA-wt-Foxo3a were found significant using Student's t-test (p < 0.01).