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Figure 4 | BMC Neuroscience

Figure 4

From: Foxo3a induces motoneuron death through the Fas pathway in cooperation with JNK

Figure 4

Constitutively active Foxo3a induces motoneuron death in a Fas-dependent manner. HA-wt-Foxo3a and HA-TM-Foxo3a were overexpressed by electroporation in control motoneurons. In parallel, HA-TM-Foxo3a was expressed in mutant motoneurons with reduced capacity for Fas signalling, lpr mice being deficient for Fas and gld mice for FasL. Cells were cultured in the presence of NTFs and grown for 2 d before counting numbers of surviving transduced motoneurons. The absence of Fas signalling reduces the ability of HA-TM Foxo3a to trigger cell death. Results are means ± SD of 4–6 wells in 2–3 independent experiments. The 100 % corresponds to the number of wt or mutant motoneurons electroporated with HA-wt-Foxo3a. Differences with the TM-Foxo3a effect in lpr and gld compared to control neurons were found significant using Student's t-test (p < 0.001 in lpr and in gld).

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